The RE1 Silencing Transcription Factor 1 Antibody from MyBioSource.com is a Rabbit Polyclonal antibody to NRSF, and REST. This antibody recognizes Human, Mouse, and Rat antigen. The RE1 Silencing Transcription Factor 1 Antibody has been validated for the following applications: Dot Blot, ELISA, and Western Blot.
Description
The mammalian neurogenesis involved a complex interaction of positive and negative regulators which can transform the neuronal stem cells into mature neurons. There are number of positive neuronal factors affecting the development of neurons have been identified and their genes are cloned through analyses of mammalian and non-mammalian embryogenesis, regeneration, repairs and in disease. The transcription factor RE1-silencing transcription factor (REST)/neuron-restrictive silencer factor (NRSF) was identified to be the first global neuronal repressor and potentially one of the critical regulators of neurogenesis (1). REST also known as NRSF is a DNA-binding protein that silences the transcription of most neuronal differentiation genes by biding to a 23bp consensus sequences known as RE1 binding site/neuron-restrictive silencer element or RE1/NRSE. The RE1 binding regions is upstream form the promoter-enhancer region of the neuronal regulatory genes (2).
REST, which is well characterized as a transcription factor that represses neuron-specific genes during embryogenesis, is switched on during middle- and late-adulthood, helping to protect neurons of the hippocampus and cortex from oxidative stress and the aggregated and misfolded proteins characteristic of Alzheimer's and other neurodegenerative diseases. The REST is expressed at high levels in most if not all neuronal cells including neuroblasts. The presence of REST in mature neurons in adult suggest a complex role for REST depending on the cellular and physiological environment. The action of REST requires interaction of several other factors such as CoREST, mSin3A and HDAC (3). Lenti virus expression of REST in NT2 cells alone caused expression of multiple neuronal differentiation genes (4).
The REST-selective antibodies were generated against peptide taken form the C-terminal end of the REST sequence. The REST antibodies are affinity purified on immobilized antigen based affinity chromatography. REST affinity purified antibodies are stabilized in antibody stabilization buffer for long-term storage